However, this method confirms only the transfer of the standards and does not provide a definitive confirmation for all the proteins on the western blot. Again, prestained standards can be used for visualization. Bio-Rad's Trans-Blot ® Turbo™ System reduces transfer protocols to as little as 3 min while maintaining high-efficiency protein transfer across a wide range of molecular weights.Īfter proteins have been transferred, the transfer efficiency should be checked visually. Traditional semi-dry transfer systems, well suited for proteins ranging from 10–100 kD, can perform transfers in 15–60 min. ![]() Protocols utilizing standard wet or tank blotting systems typically require transfer times of one hour to overnight for a broad range of protein molecular weights. When activated by UV light, these compounds covalently bind to tryptophan residues in proteins and emit a fluorescent signal that is easily detectable with stain-free enabled systems such as Bio-Rad's ChemiDoc Touch, ChemiDoc MP, and Gel Doc EZ Imaging Systems.Īfter protein separation is verified, the transfer of proteins to the western blot membrane is most commonly performed by electrophoretic transfer. This stain-free technology is exclusive to Bio-Rad and is based on proprietary trihalo compounds. TGX Stain-Free Precast Gels contain unique compounds formulated into the gel chemistry that allow immediate visualization of proteins across the whole gel without staining. Another common method is the use of prestained standards, which does not give a complete picture of protein separation. However, this involves time-consuming staining and destaining procedures. SDS-PAGE followed by Coomassie staining is a standard, widely used method to visualize and confirm protein separation in the gel. By comparison, complete electrophoretic separation can be performed at 300 V in 20 minutes with midi-format TGX gels. Typical sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) conditions for midi gels are 200 V for 30–45 minutes. The unique TGX gel formulation enables rapid separation of proteins at a high voltage while retaining Laemmli-like separation characteristics using standard sample and Tris/glycine running buffers. Includes base, 2 cassettes to hold 1-2 lunches or up to 4 mini blotter sandwiches, blotter roll.Bio-Rad offers TGX™ (Tris/glycine extended) and TGX Stain-Free™ Precast Gels for protein electrophoresis. ![]() Environmentally friendly - environmentally friendly consumables eliminate disposal costs. Flexible design - allows user to customize transfer conditions and is compatible with traditional semi-dry consumables. Superior transfer efficiency - higher transfer efficiency than other protein transfer methods. Features and Benefits of the Trans-Blot Turbo Transfer System: - Fast blot transfer - 3 minute transfers of mini or midi gels - High throughput - can transfer 1-4 mini gels or 1-2 midi gels in one operation. ![]() Consumables for protein blotting are available in ready-to-use, pre-packaged stacks or in bulk quantities requiring assembly. Trans-Blot Turbo Transfer Packs include an optimized combination of buffer, membrane, and filter paper that provides superior transfers when paired with the Trans-Blot Turbo blotting device. The system enables protein transfer in just 3 minutes without sacrificing performance compared to traditional in-tank protein transfer. ![]() The Trans-Blot Turbo Transfer System is a high performance western blotting transfer system designed to provide rapid transfers with high efficiency.
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